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A portion of the liver was excised and homogenized at 4°C using lysis buffer containing 1% TritonX100 and 100 mM Tris-HCl and 10 mM EDTA (pH 7.2) and supplemented with 1 mM phenylmethylsulfonyl-fluoride (PMSF), 2 μ g/mL aprotinin, 2 μ g/mL pepstatin, and 2 μ g/mL leupeptin as antiproteases

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